Optimized lyopreservation: tissue preserved, including viable cells
Recently optimized lyopreservation techniques have been developed. These enable the tissue to be stored at room temperature for over a year, thus can be quickly prepared and applied without the need for thawing and reconstituting. Lyoprotective agents have been shown to protect the tissue during dehydration enabling retention of viable cells, intact extracellular matrix and growth factors, thus maintaining the structure and function of the fresh tissue.
H&E (hematoxylin and eosin) staining show tissue morphology and MT (Masson’s trichrome) staining shows collagen distribution.
Cell Viability
Staining for Live and Dead Cells
Green = Live cells
Red = Dead cells
Wound healing properties of lyopreserved tissue are similar when compared to optimized cryopreservation.
- Banerjee J, Dhall S. Therapeutic benefits of treating chronic diabetic wounds with placental membrane allografts. In: Bagchi D, Das A, Roy S, eds. Wound Healing, Tissue Repair and Regeneration. Academic Press, Cambridge, MA, USA 2020:323–335.
- Dhall S, Sathyamoorthy M. Kuang JQ, et al. Properties of viable lyopreserved amnion are equivalent to viable cryopreserved amnion with the convenience of ambient storage. PLoS One. 2018;13(10):e0204060.
- Reyzelman AM, Vartivarian M, Danilkovitch A, Saunders MC. A prospective, single-center, open-label case series evaluating the clinical outcomes of lyopreserved placental membrane containing viable cells in the treatment of chronic wounds. Wounds. 2019;31(4):97-102.