Preservation human placental tissue for therapeutic use

  • Preservation techniques

Preserved placental tissue allografts

In order for placental tissue allografts to be a commercially-available on-demand therapeutic option, preservation techniques were developed to retain the components of the placenta, ensure safety by allowing time for screening and safety testing, and enable a long shelf-life.

A number of sterilization and preservation methods are currently used with the most common being dehydration and cryopreservation; however, different processes can lead to varying degrees of damage to the tissue components, potentially affecting the therapeutic potential of the tissue.

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Biology: Key Components

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Dehydration and devitalization

  1. Banerjee J, Dhall S. Therapeutic benefits of treating chronic diabetic wounds with placental membrane allografts. In: Bagchi D, Das A, Roy S, eds. Wound Healing, Tissue Repair and Regeneration. Academic Press, Cambridge, MA, USA 2020:323–335.
  2. Adds PJ, Hunt CJ, Dart JKG. Amniotic membrane grafts, “fresh” or frozen? A clinical and in vitro comparison. Br J Opthalmol. 2001;85:905-907.
  3. Banerjee J, Dhall S. Therapeutic benefits of treating chronic diabetic wounds with placental membrane allografts. In: Bagchi D, Das A, Roy S, eds. Wound Healing, Tissue Repair and Regeneration. Academic Press, Cambridge, MA, USA 2020:323–335.
  4. Cooke M, Tan EK, Mandrycky C, He H, O’Connel J, Tsend SC. Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue. J Wound Care. 2014;23(10):465–474.
  5. Johnson A, Gyurdieva A, Dhall S, Danilkovitch A, Duan-Arnold L. Understanding the impact of preservation methods on the integrity and functionality of placental allografts. Ann Plast Surg. 2017;79(2):203–213.